Determination of the activity of T-lymphocytes activated by bronchopulmonary antigens and their immunodiagnostic significance in children with bronchial asthma

ABSTRACT. It is known that under the influence of specific mitogens, sensitized T-lymphocytes produce № 199 cytokines in culture medium, which are divided into two fractions: F1 and F2. The first (F1) them determines pathogenesis AD children this fraction produced during immunopathological reactions, second (F2) responsible for immunological parameters activation T-system immunity their regulation. Goal. To investigate functional activity activated by bronchopulmonary antigens its differential immunodiagnostic value with BA. Materials methods. determine T-lymphocytes, qualitative composition fluid, discphoresis polyacrylamide gel was used 105 asthma 20 healthy aged 5 to 14 years bronchi lung tissue, as well non-specific myogen FHA from company «Reanal». Results. In BA children, more quantitative presence bands discforograms detected depending on severity course disease characterized high production F1-fraction T-lymphocytes. structural analysis cytokine disc-phorograms conditions mitogenic switched lipopolysaccharide tissue showed discrepancies form have differences determined thickness, total numbering start having serial number 8 9. Conclusions. has been established correlates expression spectrum blood serum BA, allows diagnosing accuracy.